recombinant human il 18  (R&D Systems)

Journal: iScience

Article Title: Antigen-directed single domain antibody-based TNFR1 agonists elicit preferential killing of HER2-overexpressing cancer cells

doi: 10.1016/j.isci.2026.115327

Figure Lengend Snippet: TNFR1-and HER2-specific bispecific bivalent ICMs (2 + 2) trigger reporter cell activity (A) HEK-Blue TNF reporter cells were incubated with two fixed concentrations of 10 nM and 1 nM of bsAbs, as well as 0.1 nM and 0.01 nM of (rh) TNF as a positive control and 0.1 nM of (rh) IL-18 as a negative control. After 24 h of incubation, secreted embryonic alkaline phosphatase activity was measured via OD 640 . Reporter activity was normalized to (rh) TNF signal. (B) Dose-titration of agonistic bsAbs in HEK-Blue TNF reporter cells. Mean values ±SEM of three independent experiments are shown. ∗∗∗∗ p < 0.0001,∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05 calculated by utilizing one-way ANOVA analyses and Bonferroni test.

Article Snippet: Incubation of the HEK-Blue TNF cells with 0.1 nM and 0.01 nM of TNF and IL-18 (R&D Systems, 9124-IL) were used as positive and negative control.

Techniques: Activity Assay, Incubation, Positive Control, Negative Control, Titration

Journal: Cell Reports Medicine

Article Title: Spatial omics study reveals molecular-cellular dynamics of tumor ecosystem in esophageal squamous-cell carcinoma initiation and progression

doi: 10.1016/j.xcrm.2026.102650

Figure Lengend Snippet: Characteristic changes in transcription patterns during the progression of ESCC (A) The volcano plot shows significant differences in genes between the early (ESPL and non-mESCC) and advanced (mESCC and mLN) stages of ESCC. (B) Pathway enrichment analysis of differentially expressed genes in the early and advanced stages of ESCC. (C) The gene expression levels associated with significantly enriched pathways throughout the occurrence and development of ESCC. (D) mIF staining of CCND1, LAMB1, and IL-18 on ESCC tissues. Scale bar, 200 μm. (E) Comparison of the percentages of CCND1, LAMB1, and IL-18-positive cells on ESCC tissues. Box plots show the median and interquartile range, whiskers extend to 1.5 × IQR. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, p values were calculated using a two-sided Wilcoxon rank-sum test. (F) The heatmap of gradient-changed DEGs based on pseudotime and ESCC progression of EP.

Article Snippet: IL-18 Recombinant Rabbit Monoclonal Antibody , HUABIO , Cat# HA721536; RRID: AB_3072652.

Techniques: Gene Expression, Staining, Comparison